Workshops

Sep 2024 - Sep 2024

• • Tutors: Dr Mark Poolman, Dr Dipali Singh, Pareena Verma, Dr Trunil Desai

  • In this workshop, I covered python based computation representation of metabolic network, flux balance analysis using linear programming. Programming based tool like Scrumpy was used along with Linux.

  • Linear programming approach was used to investigate the core metabolism of Staphylococcus epidermidis as practical work to determine ATP production, biofilm formation and finally identify nutrients requirements for its growth.

  • Similarly, for another practical, determination of minimal media requirements for Campylobacter jejuni was done. Here, genome-scale metabolic model of Campylobacter jejuni M1cam was done to identify minimal media requirements and also understand the antimicrobial target identification.

  •  Moreover, identification of metabolic pathways involved in AMR in E.coli using GSMM was also done as practical work. GSM of E.coli was investigated. Experimental data was integrated into GSM and reactions, were identified. Hence pathways involved in resistance against antibiotics was identified ultimately.